Fig 1: Glycoproteomic analysis identifies O-glycoproteins elevated in both Gram-positive and Gram-negative induced sepsis. (a) Schematic illustration of the experimental approach showing enrichment of core 1/3 O-glycosylated proteins by MPA lectin, and analysis by mass spectrometry. (b) List of glycoproteins identified in the core 1/3 O-linked glycans enriched septic samples. (c) MPA pulldown of pooled uninfected and septic samples followed by Western blot with anti-Fibronectin or anti-ITIH2 antibody. Entire blots and corresponding Ponceau stained membranes are shown in Figure S12. (d) Western Blot of Fibronectin and ITIH2, and Lectin Blot of MPA for sera from Gram-Positive: MRSA infected mice. (e) Western Blot of Fibronectin and ITIH2, and Lectin Blot of MPA for sera from Gram-Negative: ST infected mice. Entire blots and corresponding Ponceau stained membranes are shown in Figure S13.